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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Methods Mol Biol. 2012;821:45–58. doi: 10.1007/978-1-61779-430-8_4

Figure 1. REDD1 is required for inhibition of mTORC1 activity under hypoxia.

Figure 1

Hypoxia inhibits mTORC1 activity in wild-type but not REDD1-/- MEFs, as evidenced by dephosphorylation of S6K (T389) and 4E-BP1 (T70). MEFs of each genotype growing in 10% serum were exposed to hypoxia (1% O2) for the indicated times. The same blot was stripped and reprobed for the respective total proteins. Note the prominence of hypophosphorylated 4E-BP1 (lower band) upon hypoxic exposure of wild-type cells. Beta tubulin serves as a loading control. Adapted from Genes Dev. 22:239.