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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Methods Mol Biol. 2012;821:45–58. doi: 10.1007/978-1-61779-430-8_4

Figure 2. REDD1 is required for hypoxia-induced TSC2/14-3-3 dissociation.

Figure 2

MEFs of the indicated genotype were treated with hypoxia (3 hours) followed by western analysis or IP for endogenous 14-3-3. Hypoxia-induced TSC2/14-3-3 dissociation and S6K1 (T389) dephosphorylation are both absent in REDD1-/- MEFs. Adapted from Genes Dev. 22:239.