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. Author manuscript; available in PMC: 2014 Mar 19.
Published in final edited form as: Methods Mol Biol. 2012;821:45–58. doi: 10.1007/978-1-61779-430-8_4

Table 1. Antibody reagents and suppliers.

Antibody Supplier Product no. Recommended use
Phospho-4E-BP1 (Thr70) Cell Signalling Technologies #9455 (WB) 1:1,000 1% BSA-PBST O/N
Phospho-p70S6K (Thr389) Cell Signalling Technologies #9205 (WB) 1:1,000 1% BSA-PBST O/N
Phospho-S6 (Ser235/Ser236) Cell Signalling Technologies #2211 (WB) 1:1,000 1% BSA-PBST O/N
4E-BP1 Santa Cruz Biotechnology #6936 (WB) 1:1,000 5% milk-PBST 1h
p70S6K Cell Signalling Technologies #9202 (WB) 1:1,000 5% milk-PBST 1h
S6 Cell Signalling Technologies #2217 (WB) 1:1,000 5% milk-PBST 1h
β-Tubulin Millipore #MAB 3408 (WB) 1:10,000 5% milk-PBST 1h
Pan-14-3-3 Thermo Scientific #MS-1504P (IP) 1ug/mg protein, (WB) 1:1,000 5% milk-PBST O/N
TSC2 Santa Cruz Biotechnology #893 (WB) 1:1,000 5% milk-PBST O/N
Goat α-mouse IgG-HRP Biorad #170-6516 (WB) 1:5000 either 1% BSA or 5% milk-PBST 1h
Goat α-rabbit IgG-HRP Biorad #170-6515 (WB) 1:5000 either 1% BSA or 5% milk-PBST 1h