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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2013 Sep 24;133(2):379–387.e1. doi: 10.1016/j.jaci.2013.07.037

Figure 5. PGE2 inhibits IL-13 production primarily by inhibiting T cells through EP2.

Figure 5

(A) and (A) EP2 deficiency on T cells primarily contributes to enhanced Th2 responses in EP2−/− mice. Purified DCs and T cells were co-cultured at a ratio of 1:10 for 3 days in the presence of 100 μg/ml OVA. Data are from one experiment representative of 3 independent experiments. (B) CD4pos T cells from EP2−/− mice exhibited significantly higher IL-13 production than did cells from wild type mice (C) PGE2 suppresses Th2 polarization. Purified CD4pos T cells were cultured in the presence of 1 μM PGE2, EP2, EP3, or EP4 agonist or 500 μM PKA-specific cAMP analog 6-Bnz-cAMP or the Epac-specific cAMP analog 8-pCPT-2′-O-Me-cAMP. Treatments statistically different compared to control are indicated with (#). (n=3) ** p<0.01, *** p<0.001.