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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jan 14;69:67–76. doi: 10.1016/j.freeradbiomed.2014.01.010

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Selenoproteins in the urine of megalin−/− and megalin+/+ mice. Panels A and C are autoradiograms of SDS-PAGE gels of urine and plasma samples from 75Se-labeled megalin−/− and megalin+/+ mice. Panels B and D are the Coomassie stains of the respective gels depicted in panels A and C. Lane 1 in panels A and B was loaded with 1 μl megalin+/+ urine (1660 cpm 75Se) and lane 2 with 20 μl of the same urine after dialysis (1500 cpm 75Se). Lane 3 was loaded with 5 μl megalin−/− urine (2160 cpm 75Se) and lane 4 with 10 μl of the same urine after dialysis (1880 cpm 75Se). Lane 1 in panels C and D was loaded with 10 μl dialyzed megalin−/− urine (2060 cpm 75Se). The dialyzed megalin−/− urine was passed over a monoclonal antibody (9S4) column that binds the N-terminal domain of Sepp1. The flow through was loaded onto lane 2 and the bound fraction was loaded onto lane 3. Lanes 4 and 5 were each loaded with 1 μl plasma from megalin+/+ (1890 cpm 75Se) and megalin−/− (2170 cpm 75Se) mice, respectively.