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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jan 14;69:67–76. doi: 10.1016/j.freeradbiomed.2014.01.010

Table 2.

PCR primers for genotyping

primer
designation		 primer sequence (5’-< 3’) PCR product size
(pb)
Sepp1 SePS14 GCCATCAGGGCTCAGTGCAG wildtype: 600
(SePS14/SePA600)
SePA600 GGTGTTGGGTCGTTTGTTCGGATCG
NeoS2 GGATGCCTGCAGAAGACACAAGTA homozytote: 500
(SePS14/NeoS2)
Sepp1 □240-
361 		MoSePS18 GCAGGAGCATCTTGGCAGCAGT mutant: 450
wildtype: 370
MoSePS19 CACTGACAGGATTCAGTGACTTTCTCCT
Sepp1U40S WS1004 TCCTAGATTGGCAGAGGATAGAATGAA mutant: 198
wildtype: 116
WS1005 TCAGAAACACCTTCCAACTGTAATGC
megalin G20 GCACATTTGGCCAGCCAAGG wildtype: 275
(G20/G21)
G21 CATATCTTGGAAATAAAGCGAC
S175 GATTGGGAAGACAATAGCAGGCATGC homozygote: 325
(G21/S175)