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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jan 14;69:77–85. doi: 10.1016/j.freeradbiomed.2014.01.011

Figure 3. Effects of RTV and high Mg on (A) BAEC nitrite+nitrate levels and on (B) eNOS mRNA expression.

Figure 3

BAECs were incubated with 15µM RTV with normal (0.8mM) or high Mg (2 mM) for 24hr. Supernatants were retrieved for total NO products (nitrite + nitrate) determination by the Griess Reagent. (B): Real time quantitative-PCR was performed to measure eNOS mRNA level in BAECs. Bovine 18s rRNA was used as the reference gene. Expression levels were compared to control group. Each assay was repeated in triplicate and data represent means ±SEM, n=4–5.; *p<0.05, **p<0.01 vs controls; +P<0.05, ++P<0.01 vs RTV (in 0.8 mM Mg).