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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: FEBS J. 2014 Feb 13;281(6):1571–1584. doi: 10.1111/febs.12724

Figure 1. SSTY proteins are specifically present in round and elongating spermatids.

Figure 1

Representative pictures of immunodetection using SSTY1/2 antibody on testicular sections from WT and MSYq− mice, at stages II–V, VI–VII and VIII–XI. Black and white pictures represent DAPI staining of the nuclei. The different cell types present in testes are indicated: spermatocytes at the pachytene stage (P), round spermatids (RS), elongating spermatids (ES) and condensing spermatids (CS). Anti-SSTY1/2 was detected in green. Lectin-PNA (red) was used to stain acrosomes for staging purposes. The specificity of SSTY1/2 signal was controlled on testicular sections from mutant mice (MSYq−) which do not express SSTY (thus considered as a negative control, ctl-). SSTY proteins are observed in the round spermatids and elongating spermatids, from stage II until stage XI. Scale bar indicates 20μm. See also supplemental Fig. S2 for extended negative control panels and comparison of the pattern of expression of SSTY in WT and shSLY mice.