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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: FEBS J. 2014 Feb 13;281(6):1571–1584. doi: 10.1111/febs.12724

Figure 2. SSTY proteins are enriched in subnuclear regions of the spermatids.

Figure 2

Representative pictures of immunodetection of SSTY proteins in the nucleus of WT, shSLY and MSYq− spermatids, taken at a higher magnification (x60); (A) using anti-SSTY1 antibody or (B) using anti-SSTY1/2 antibody. As negative controls, both antibodies were used on MSYq− testicular sections (tissue which does not express SSTY) and anti-SSTY1/2 pre-immune serum was used on shSLY sections. Anti-SSTY1/2, anti-SSTY1 or pre-immune serum were detected in green. Lectin-PNA (red) was used to stain acrosomes for staging purpose. Black and white pictures represent DAPI staining of the nuclei. The most DAPI-dense round-shaped structure is the chromocenter; the less DAPI-dense region near the chromocenter is the postmeiotic sex chromatin (indicated by white arrowheads). SSTY protein staining is not diffuse but enriched in some subnuclear regions, among which is the postmeiotic sex chromatin (PMSC, white arrowheads). Scale bar indicates 10μm.