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. 1969 Jan;44(1):95–100. doi: 10.1104/pp.44.1.95

Selective Inhibition by Actinomycin D of the Synthesis in Photosynthetic and Non-photosynthetic Enzymes During the Greening of Etiolated Bean Leaves

B Andrea Melandri 1,2, Assunta Baccarini 1,2, Giorgio Forti 1,2
PMCID: PMC396044  PMID: 16657039

Abstract

The effect of actinomycin D on the synthesis of the photosynthetic apparatus during illumination of etiolated leaves of Phaseolus vulgaris was studied. The increase of chlorophyll content and of the activities of some photosynthetic enzymes (NADPH diaphorase, ferredoxin, NADP+ glyceraldehyde-3-phosphate dehydrogenase) was compared with simultaneous measurements of the level of other enzymes not considered associated with photosynthesis (ornithine transcarbamylase, glucose-6-phosphate dehydrogenase, NAD+ glyceraldehyde-3-phosphate dehydrogenase).

The effect of the inhibitor on the synthesis of the components of the photosynthetic apparatus is much larger than its effect on the synthesis of non-photosynthetic enzymes when the antibiotic is supplied 2 hr before illumination. The same selective action is also obtained if actinomycin D is added after 20 hr of exposure of the leaves to light.

The markedly different sensitivity to the inhibitor of the synthesis of photosynthetic enzymes, as compared to non-photosynthetic ones, is interpreted as a selective inhibition at the level of DNA-directed synthesis of RNA molecules.

This RNA may be involved either in the regulation of chloroplast differentiation or in the specification of some component essential for the formation of the plastidial structure or for the activity of plastidial ribosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen S., McMahon D., Bogorad L. Early effects of illumination on the activity of some photosynthetic enzymes. Plant Physiol. 1967 Jan;42(1):1–5. doi: 10.1104/pp.42.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gibbs S. P. Synthesis of chloroplast RNA at the site of chloroplast DNA. Biochem Biophys Res Commun. 1967 Aug 23;28(4):653–657. doi: 10.1016/0006-291x(67)90364-6. [DOI] [PubMed] [Google Scholar]
  4. Givan A. L., Levine R. P. The photosynthetic electron transport chain of Chlamydomonas reinhardi. VII. Photosynthetic phosphorylation by a mutant strain of Chlamydomonas reinhardi deficient in active P700. Plant Physiol. 1967 Sep;42(9):1264–1268. doi: 10.1104/pp.42.9.1264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KEISTER D. L., JAGENDORF A. T., SAN PIETRO A. Development of bean-leaf transhydrogenase in etiolated leaves. Biochim Biophys Acta. 1962 Aug 13;62:332–337. doi: 10.1016/0006-3002(62)90047-1. [DOI] [PubMed] [Google Scholar]
  6. Keller C. J., Huffaker R. C. Evidence for in vivo Light-induced Synthesis of Ribulose-1,5-diP Carboxylase and Phosphoribulokinase in Greening Barley Leaves. Plant Physiol. 1967 Sep;42(9):1277–1283. doi: 10.1104/pp.42.9.1277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Levine R. P., Gorman D. S. Photosynthetic electron transport chain of Chlamydomonas reinhardi. 3. Light-induced absorbance changes in chloroplast fragments of the wild type and mutant strains. Plant Physiol. 1966 Oct;41(8):1293–1300. doi: 10.1104/pp.41.8.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MEGO J. L., JAGENDORF A. T. Effect of light on growth of Black Valentine bean plastids. Biochim Biophys Acta. 1961 Oct 28;53:237–254. doi: 10.1016/0006-3002(61)90437-1. [DOI] [PubMed] [Google Scholar]
  10. Marcus A. Photocontrol of Formation of Red Kidney Bean Leaf Triphosphopyridine Nucleotide Linked Triosephosphate Dehydrogenase. Plant Physiol. 1960 Jan;35(1):126–128. doi: 10.1104/pp.35.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Margulies M. M. Effect of Chloramphenicol on Light-Dependent Synthesis of Proteins and Enzymes of Leaves and Chloroplasts of Phaseolus vulgaris. Plant Physiol. 1964 Jul;39(4):579–585. doi: 10.1104/pp.39.4.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Margulies M. M. Relationship Between Red Light Mediated Glyceraldehyde-3-Phosphate Dehydrogenase Formation and Light Dependent Development of Photosynthesis. Plant Physiol. 1965 Jan;40(1):57–61. doi: 10.1104/pp.40.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Richards O. C. Hybridization of Euglena gracilis chloroplast and nuclear DNA. Proc Natl Acad Sci U S A. 1967 Jan;57(1):156–163. doi: 10.1073/pnas.57.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scott N. S., Smillie R. M. Evidence for the direction of chloroplasts ribosomal RNA synthesis by chloroplast DNA. Biochem Biophys Res Commun. 1967 Aug 23;28(4):598–603. doi: 10.1016/0006-291x(67)90355-5. [DOI] [PubMed] [Google Scholar]
  15. Smillie R. M., Graham D., Dwyer M. R., Grieve A., Tobin N. F. Evidence for the synthesis in vivo of proteins of the Calvin cycle and of the photosynthetic electron-transfer pathway on chloroplast ribosomes. Biochem Biophys Res Commun. 1967 Aug 23;28(4):604–610. doi: 10.1016/0006-291x(67)90356-7. [DOI] [PubMed] [Google Scholar]
  16. Tewari K. K., Wildman S. G. DNA polymerase in isolated tobacco chloroplasts and nature of the polymerized product. Proc Natl Acad Sci U S A. 1967 Aug;58(2):689–696. doi: 10.1073/pnas.58.2.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tewari K. K., Wildman S. G. Function of chloroplast DNA. I. Hybridization studies involving nuclear and chloroplast DNA with RNA from cytoplasmic (80S) and chloroplast (70S) ribosomes. Proc Natl Acad Sci U S A. 1968 Feb;59(2):569–576. doi: 10.1073/pnas.59.2.569. [DOI] [PMC free article] [PubMed] [Google Scholar]

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