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. 2014 Mar 1;4(2):135–147.

Figure 4.

Figure 4

Sepantronium damages DNA. Immunofluorescence staining of γH2AX (green) in H1299 and H661 cells treated with DMSO, sepantronium and etoposide. Red color is tubulin counterstaining (A). Quantitative real time PCR results showing induction of BAX and XRCC5 RNA at 2 hours and CDKN1A at 4 hours (B). GAPDH was used as control. (C) Comet assay images of H1299 treated with DMSO, sepantronium and etoposide. (D) Quantitation of tail length, tail area, %DNA in tails and tail moment performed with software. (E) Analysis of checkpoint phospho protein by Western blot. *P<0.05, **P<0.01, ***P<0.001.