Figure 1.

GNG2 inhibits migration and invasion of SK-Mel28 cells. (A) Levels of GNG2 protein expression in stable clones of SK-Mel28 cells with empty (Clones 1-3) or GNG2 expression (Clones 5, 6 and 13) vectors are presented. Two stable clones (Clones 6 and 13) were used in subsequent experiments. (B) Wound healing assay was performed with control (Clone 1) and GNG2-overexpressed (Clones 6 and 13) SK-Mel28 cells. Cultured cells were scratched with a sterile pipette tip and evaluated after incubation for 16 hrs. (C, D) Cell migration (top panels in C; left panel in D) and invasion (bottom panels in C; right panel in D) in vitro were examined by the Boyden chamber assay and Matrigel invasion assay, respectively. Morphology (C) and index as percentages (D; mean ± SE) of migrating and invading cells after incubation for 16 hrs are shown. **, Significantly different (**, p<0.01) from the control (Clone 1) by Student’s t-test.