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. 2014 Mar 19;34(12):4135–4147. doi: 10.1523/JNEUROSCI.3779-13.2014

Figure 7.

Figure 7.

Vesicles containing dendritic proteins undergo selective transport before axon specification. A, B, Phase contrast and fluorescence images of a Stage 2 cell expressing CA-Kinesin-1-tdTomato (red) and GFP-rab11b (green). CA-Kinesin-1 accumulated strongly at the tips of neurites 1 and 3. The corresponding video is shown in Movie 5. C, Anterograde events traced from kymographs show the anterograde transport of GFP-rab11b in each neurite. The kymographs outlined in red are taken from neurites 1 and 3, which contain high amounts of CA-Kinesin-1. The percentage of total CA-Kinesin-1 fluorescence accumulated in each neurite is indicated above the kymographs. D, The relationship between the fraction of CA-Kinesin accumulated in each neurite (x-axis) and the amount of rab11 transport in the same neurite (y-axis). Neurites that contained significant amounts of CA-Kinesin-1 fluorescence (>20%) had significantly lower amounts of GFP-rab11b transport. Each symbol corresponds to a single neurite. Contrast in images was nonlinearly adjusted to improve visibility of dim moving vesicles. Neurons were imaged at 1 DIV; n = 10 neurons. Scale bar, 20 μm.