Figure 3.

ALS-linked TDP-43 mutations exhibit a higher proportion of slower moving RNA granule trafficking. A, Velocities of transfected TDP-43::GFP were quantified by measuring the change in position of a granule between two consecutive time-lapse images and dividing the change of distance by the time between images. More than 500 RNA granules from nine different cells were used for quantification. B, C, Velocity distribution of TDP-43::GFP-enriched RNA granules. The velocities from A were categorized into two groups, >0.1 μm/s and <0.1 μm/s. ALS-linked mutations reduced the number of RNA granules trafficking with velocities >0.1 μm/s.