Figure 4.

Localization of TDP-43 after KCl-induced depolarization of hippocampal neurons. Cultured rat hippocampal neurons were transfected with TDP-43::GFP (WT, A315T, or Q343R). Twenty-four hours after transfection, the neurons were treated with TTX (1 μm) for 1 h, pulsed with 90 mm KCl 4 × 3 min, allowed to incubate for 6 h, and then GFP was imaged directly. A–C, Representative images of TDP-43::GFP (WT, A315T, and Q343R) in the hippocampal neurons. D–F, Tracings of TDP-43 present in the processes from images from A–C. G, Quantification of distance traveled by WT, A315T, and Q343R TDP-43. H, Quantification of the number of neurites per neuron exhibiting evidence of TDP-43 (WT, A315T, and Q343R) puncta in the soma or dendritic arbor. Scale bar, 10 μm.