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Overexpression of Cdk5 coactivator p35 blocks KCN-induced Kv2.1 channel declustering in cortical neurons. Top, Neurons were transfected with GFP-Kv2.1 and p35 or vector, preconditioned 24 h after transfection, and imaged immediately following preconditioning. Scale bar, 10 μm. Bottom, Number of clusters per cell was counted and averaged for 15–39 cells per group from six independent experiments (mean ± SEM). *p < 0.05 (ANOVA/Bonferroni's test vs vehicle).
