Figure 6.

(A) Experimental timeline. Primary neurons were cultivated for 2 weeks in high (3.5 mM) Mg²⁺ medium and treated with BSA (control), BDNF or TrkB-Fc in low (1.5 mM) Mg²⁺. (B) Graph comparing the frequency of calcium transients in hippocampal primary neurons cultivated in high or low Mg²⁺ containing medium. (C) Graph comparing the amplitude of calcium transients in hippocampal primary neurons cultivated in high or low Mg²⁺ containing medium. (D) Histogram of the total number of intersections in hippocampal primary neurons cultivated in high or low Mg²⁺ containing medium. (E) Graphs comparing dendritic spine density between neurons cultivated in low or high Mg²⁺ and treated with BSA (control), BDNF and TrkB receptor bodies (TrkB-Fc). (F) Histogram comparing the spine head width between primary hippocampal neurons cultivated in high Mg²⁺ and treated with BSA (control), BDNF and TrkB receptor bodies (TrkB-Fc). (G) Histogram of the dendritic spine length between primary hippocampal neurons cultivated in high Mg²⁺ and treated with BSA (control), BDNF and TrkB receptor bodies (TrkB-Fc). (H) Graph showing the binning of spines according to their spine head width and comparing the proportion of spines within each category in response to a BSA (control), BDNF or BDNF antibodies (BDNF-Abs) treatment. (I) Graph showing the binning of spines according to their spine length and comparing the proportion of spines within each category in response to a control, BDNF or TrkB receptor bodies (TrkB-Fc) treatment. The number in the columns represents the number of cells analyzed. Significance is indicated as follows: ^*p < 0.05; ^***p < 0.001. Error bars indicate s.e.m. Unpaired two-tailed Student Test and ANOVA, post-hoc Tukey's Multiple Comparison Test.