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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Yeast. 2013 Dec 13;31(3):103–112. doi: 10.1002/yea.2992

Figure 1.

Figure 1

Plasmid pJH136, a template for URA3 cassette PCR. The U2 and D2 sequences provide robust PCR amplification of the 1160 bp cassette. URA3.for and URA3.rev primers can be used for split-URA3 PCR, if needed (see Materials and Methods).