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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Yeast. 2013 Dec 13;31(3):103–112. doi: 10.1002/yea.2992

Figure 4.

Figure 4

Gel analysis of 50/50 cassette PCR and genomic PCR of yeast strains created in this study. Marker (M) sizes are given in bp. (A) 50/50 PCR cassettes for yeast transformation. ExTaq was used for the 50 μL PCRs, of which 4 μL were loaded on the gel. On the left are single PCRs of the full 50/50 URA3 cassettes. On the right are split-URA3 PCRs performed with the same primers but in combination with URA3.for and URA3.rev. Either single or pooled split-URA3 PCRs can be used for yeast transformation. (B) Genomic PCR of MATALPHA1 alleles. Expected sizes (bp): wildtype (1278), α1-2x (1276), α1∆ (750), wildtype with XhoI (1278), α1-2x with XhoI (882, 394).