Skip to main content
. 2014 Mar 20;5:108. doi: 10.3389/fimmu.2014.00108

Figure 3.

Figure 3

Oct2 is required for normal signaling from the B cell receptor, and ectopic Syk expression enhances the response in both WT and KO B cells. (A) B cell proliferation in response to BCR signaling, Baff, or both in combination after 3 days. Stimulation index is calculated as proliferation relative to unstimulated cells. Filled bars, Oct2+/+ gray bars, Oct2−/−. All values are the mean of triplicates ± SD. (B) Survival, assessed by propidium iodide exclusion, of B cells cultured with Baff or with anti-μ for 3 days. Filled bars, Oct2+/+ gray bars, Oct2−/−. All values are the mean of triplicates ± SD. (C) Syk protein levels in cloned B lymphoma cells transduced with a Syk-expressing retrovirus. The Oct2+/+ and Oct2−/−cell lines are BC1 and OM1, respectively (48). (D) Cytometric measurement of Ca2+ flux in clones of BC1 and OM1 cells transduced with vector only (black) or a Syk-expressing retrovirus (red). The arrow indicates the timing of addition of anti-μ to cross-link the BCR. (E) Primary splenic B cells from Oct2+/+ and Oct2−/−mice were activated, transduced (see Materials and Methods) and subsequently treated with anti-μ. The number of live, transduced (GFP+) cells in each culture after 48 h is shown. Values are means (n = 4) ± SD. Filled bars, Oct2+/+ gray bars, Oct2−/−.