Figure 4.
Differences in compositions and structures of mixed-species biofilms grown at different positions of the flow cell. (a) Confocal microscopy images showing mixed-species biofilms of PAO1 (yellow), Pf-5 (cyan) and KP-1 (red) growing at the inlet end and outlet end of the flow cell. The top and side images represent the x-z and y-z planes respectively. The green and red lines indicate the positions corresponding to the x-z and y-z cross sections respectively. The blue line indicates the x-y plane of the main panel. Magnification × 20. Scale bar: 50 μm. (b) Bar chart depicting the percentages of PAO1, Pf-5 and KP-1 within the mixed-species biofilms grown at the inlet end and outlet end of the flow cell. Error bars represent standard deviations (n=3). * denotes significant difference (One-way ANOVA: P-value<0.05).