| ColE1_F |
5′AGGATCCCCGGGGATAACGCAGGAAAGAACAT3′ |
Primer used during PCR amplification of ColE1 ori. Primer is flanked with SmaI site at 5′ end. |
| ColE1_R |
5′GATTACGAATTCCTGTCAGACCAAGTTTACTC3′ |
Primer used during PCR amplification of ColE1 ori. Primer is flanked with EcoRI site at 5′ end. |
| Tn7R |
5′CAGCATAACTGGACTGATTTCAG3′ |
Common primer used for checking chromosomal insertion of Tn7. |
| PAglmS-down |
5′GCACATCGGCGACGTGCTCTC3′ |
Primer used with Tn7R to check chromosomal insertion of Tn7 in PAO1. |
| PF_tn7_R2 |
5′TGCCGCACATCCACGACATCCTC3′ |
Primer used with Tn7R to check chromosomal insertion of Tn7 in Pf-5. |
| KP_tn7_R2 |
5′GTGGCGCCGAACAACGAACTGCT3′ |
Primer used with Tn7R to check chromosomal insertion of Tn7 in KP-1. |
