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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Cancer Res. 2013 Feb 1;11(4):418–426. doi: 10.1158/1541-7786.MCR-12-0518

Figure 4. EpCAM modulates IL-8 promoter activity and NF-κB transcription factor activity.

Figure 4

(A) A schematic diagram of the IL-8 promoter is indicated, demonstrating AP-1, C/EBPβ, and NF-κB binding sites. (B) MCF-7, MDA-231, and CA1a breast cancer cells were stably transduced with lentiviral shRNA constructs targeting EpCAM (shUTR) or an irrelevant nucleotide sequence (shSCR). To measure IL-8 promoter activity, transduced cells were transfected with constructs containing the IL-8 promoter fused to luciferase, and a transfection control construct, TK-renilla. Relative luciferase activity was analyzed using a dual-luciferase kit. (C) MDA-231 cells transduced with the indicated lentiviral constructs were transfected with non-promoter luciferase reporter constructs for AP-1, C/EBPβ, NF-κB, and transfection control TK-renilla. Relative luciferase activity was analyzed using a dual-luciferase kit (Promega). Asterisks indicate significance at P value <0.05 when compared to control.