Figure 6.
Effect of genistein on sFRP1 and Smad4 genes in prostate cancer cells. (A) sFRP1 and Smad4 expression in DMSO (control) and genistein 25 μM (G25) treated prostate cancer cells. (B) Representative bisulphite modified genomic DNA sequencing of the sFRP1 promoter region from control and genistein-treated PC-3 cells. (C) Representative bisulphite modified genomic DNA sequencing of the Smad4 promoter region from control and genistein-treated PC-3 cells. (D) Quantification of ChIP real-time PCR analysis in PC-3 cells in sFRP1 and Smad4 genes promoter region in control and genistein-treated 786-O cells.