Table 1. Properties of currently available next-generation sequencing technologies.
| Platform | Read length | Throughput/ run |
Approximate time/run |
Machine cost (US$) |
Reagent cost (US$) |
Reagent cost/Gb (US$) |
Primary error |
Base error rates |
|---|---|---|---|---|---|---|---|---|
| HiSeq™ 2000 | 100 bp | 600 Gb | 11 days | 690,000 | 23,470 | 40 | Substitution | ~1–2% over 100 bp |
| SOLiD™ 4 | 75 bp | 100 Gb | 12 days | 475,000 | 8128 | <110 | A–T bias | 0.06% |
| SOLiD™ 4hq | 75 bp | 300 Gb | 14 days | 595,000 | 10,503 | 70 | A–T bias | 0.01% |
| SOLiD™ PI | 75 bp | 77 Gb | 8 days | 349,000 | 6101 | 80 | A–T bias | 0.01% |
| 454GSFLX Titanium XL+ | 700 mean bp, ≤1000 bp | 700 Mb | 23 h | 500,000 | 6200 | 7000 | Indel | 0.5% |
| lonTorrent™ PGM™ 316 | 200 bp | 100 Mb | ~2 h | 50,000 | 750 | <7500 | Indel | 1.2% over 150 bp |
| lonTorrent™ PGM™ 318 | 200 bp | 1 Gb | ~2 h | 50,000 | 925 | <925 | Indel | 1.2% over 150 bp |
| MiSeq® | 150 bp | >1 Gb | 27 h | 125,000 | 750 | 740 | Substitution | ~1–2% over 100 bp |
| 454 GS Junior | 400 mean bp | 35 Mb | 12 h | 108,000 | 1100 | 22,000 | Indel | 1.00% |
| PacBioRS (early 2012) | 2700 mean bp, ≤5000 bp | 90 Mb per cell | <1 day (?) | 695,000 | 110–1700 | 11,000- 340,000 |
CG deletion | 13.00% |
Currently available next-generation sequencing technologies. All specifications are liable to rapid change. Cost information is taken from Glenn [18], but can vary widely by country and individual deal. The read lengths given are taken from manufacturers websites [101-105], and are the maximum over all currently available protocols with the system in question. The same holds for throughput, and times are the shortest for the given throughput. Base error rate, from the same sources, should be taken as a rough indicator only as the details of errors vary. It is an average for the read, or for a fixed number of bases from the start of the read (which is stated in this case).