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Study design for the development of the ovalbumin (OVA)-induced asthma model using Sprague-Dawley rats. Animals were sensitized with OVA-alum injections on days 0, 7, and 14 followed by challenges with allergen on days 15, 16, and 17. The treatments were administered once a day 1 hr before the OVA challenges, and blood, BALF, and lung tissue samples were collected as shown in this figure.
