Figure 4. Maximal Th1 cell stimulation by LPS requires T cell intrinsic expression of IL-18R and IL-33R.
Mixed BM chimeras were generated, infected, and stimulated, as described in Figure 3. A graph showing the percentage of IFN-γ+ Th1 cells in wild-type versus gene-deficient CD4+ T cells is shown for (A) Il1r1, (B) Ifngr1, (C) Il18r1, and (D) Il1rl1 (IL33R) mixed BM chimeras. All experiments include at least 3 mice per group and were performed at least twice with similar results. Statistical analyses were performed by two-way repeated measures ANOVA with bonferroni post-test. Error bars represent mean +/− SEM. See also Figure S3.
