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. 2013 Jun 28;2(3):861–871. doi: 10.3390/biology2030861

Figure 3.

Figure 3

Determination of miR-EMT levels in cells by qPCR analysis. (A) Levels of miR-EMT increased approximately 10.000-fold in LA7 cells transfected with 100 nM of miR-EMT Mimic (miR-EMT-Mimic) compared to cells transfected with 100nM scrambled-Mimic (scr-Mimic). The same level of miR-EMT was also detected in LA7 cells transfected with miR-EMT-Mimic oligos and transduced “miR-EMT-scavenger” (miR-EMT-Scavenger + miR-EMT-Mimic) suggesting that the mode of miR-EMT scavenger action is not to eliminate but to sequester miR transcripts in cells. Plot is represented in logarithmic scale using U6 snRNA as an endogenous control. (B) Detail of the up-regulation of miR-EMT expression in miR-EMT-scavenger-expressing cells; plot is represented in linear scale using U6 snRNA as an endogenous control. Error bars represent three standard deviations with 95% of confidence. The results are representative of three studies with a p < 0.05.