. 2013 Apr;10(2):98–107. doi: 10.1513/AnnalsATS.201209-080OC

Table 1.

Coagulation proteins detected by liquid chromatography-tandem mass spectrometry protein profiling of plastic bronchitis airway casts

Identified Proteins	Accession Number	Molecular Weight (kD)	Cast 1	Cast 2	Cast 3
Fibrinogen γ chain	P02679	52	0.04537	0.03212	0.00541
Fibrinogen β chain	P02675	56	0.03990	0.03343	0.00247
Fibrinogen α chain	P02671	95	0.03099	0.03548	0.00570
α₁-Antitrypsin	P01009	47	0.01627	0.00825	0.03670
α₂-Macroglobulin	P01023	163	0.01088	0.00939	0.01328
Plasminogen	P00747	91	0.00395	0.00287	0.00131
α₂-Antiplasmin	P08697	55	0.00356	0.00387	0.00119
Fibronectin	P02751	263	0.00296	0.00722	0.00089
Antithrombin-III	P01008	53	0.00296	0.00274	0.00205
Prothrombin	P00734	70	0.00142	0.00097	0.00194
Vitamin K–dependent protein S	P07225	75	0.00066	0.00044	0.00024
Plasma kallikrein	P03952	71	0.00032	0.00000	0.00046
Coagulation factor XIII A chain	P00488	83	0.00027	0.00187	0.00000
Coagulation factor XIII B chain	P05160	76	0.00025	0.00003	0.00006
Coagulation factor XII	P00748	68	0.00006	0.00000	0.00032
Coagulation factor X	P00742	55	0.00004	0.00000	0.00011
Plasminogen activator inhibitor-2	P05120	47	0.00002	0.00000	0.00000
von Willebrand factor	P04275	309	0.00002	0.00007	0.00011
Coagulation factor V	P12259	252	0.00001	0.00000	0.00002
Coagulation factor IX	P00740	52	0.00000	0.00000	0.00006
Coagulation factor XI	P03951	70	0.00000	0.00000	0.00004
Vitamin K–dependent protein C	P04070	52	0.00000	0.00000	0.00008
Thrombospondin-1	P07996	129	0.00000	0.00000	0.00023
Plasminogen activator inhibitor-1	P05121	45	0.00000	0.00000	0.00004

Data are the normalized spectral abundance factor (NSAF) rounded to five decimal places of each detected protein in each of three plastic bronchitis airway casts in order of abundance based on cast 1; plasmin cannot be discriminated from plasminogen and activated coagulation factors cannot be discriminated from the zymogen. The NSAF was calculated using the equation: NSAF = (SpC/MW)/Σ(SpC/MW)_N, where SpC = spectral counts, MW = protein molecular weight (kD), and N = total number of proteins.