Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1969 Mar;44(3):377–384. doi: 10.1104/pp.44.3.377

Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations 1

Stephen A Bard a, Milton P Gordon a
PMCID: PMC396095  PMID: 5775204

Abstract

A method for the large-scale preparation of spinach chloroplasts using the Spinco Model L-4 zonal ultracentrifuge and for the extraction of DNA from the chloroplasts is described. Thirty-five percent of the chloroplast DNA (ρ = 1.706 g/cc) differs from nuclear DNA (ρ = 1.695 g/cc) in buoyant density. Tm, base composition, and renaturation properties. Sixty-five percent of the chloroplast DNA (ρ = 1.696 g/cc) has the same buoyant density and Tm as nuclear DNA, but it differs in base composition and renaturation properties.

Full text

PDF
384

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  2. BRAWERMAN G., EISENSTADT J. M. DEOXYRIBONUCLEIC ACID FROM THE CHLOROPLASTS OF EUGLENA GRACILIS. Biochim Biophys Acta. 1964 Nov 15;91:477–485. doi: 10.1016/0926-6550(64)90077-5. [DOI] [PubMed] [Google Scholar]
  3. Boardman N. K., Francki R. I., Wildman S. G. Protein synthesis by cell-free extracts of tobacco leaves. 3. Comparison of the physical properties and protein synthesizing activities of 70 s chloroplast and 80 s cytoplasmic ribosomes. J Mol Biol. 1966 Jun;17(2):470–487. doi: 10.1016/s0022-2836(66)80157-2. [DOI] [PubMed] [Google Scholar]
  4. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  5. CHUN E. H., VAUGHAN M. H., Jr, RICH A. THE ISOLATION AND CHARACTERIZATION OF DNA ASSOCIATED WITH CHLOROPLAST PREPARATIONS. J Mol Biol. 1963 Aug;7:130–141. doi: 10.1016/s0022-2836(63)80042-x. [DOI] [PubMed] [Google Scholar]
  6. Granick S., Gibor A. The DNA of chloroplasts, mitochondria and centrioles. Prog Nucleic Acid Res Mol Biol. 1967;6:143–186. doi: 10.1016/s0079-6603(08)60526-7. [DOI] [PubMed] [Google Scholar]
  7. Green B. R., Gordon M. P. The satellite DNA's of some higher plants. Biochim Biophys Acta. 1967 Sep 26;145(2):378–390. doi: 10.1016/0005-2787(67)90056-1. [DOI] [PubMed] [Google Scholar]
  8. POLLARD C. J. THE DEOXYRIBONUCLEIC ACID CONTENT OF PURIFIED SPINACH CHLOROPLASTS. Arch Biochem Biophys. 1964 Apr;105:114–119. doi: 10.1016/0003-9861(64)90241-3. [DOI] [PubMed] [Google Scholar]
  9. Parenti F., Margulies M. M. In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing Leaves. Plant Physiol. 1967 Sep;42(9):1179–1186. doi: 10.1104/pp.42.9.1179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tewari K. K., Wildman S. G. Chloroplast DNA from tobacco leaves. Science. 1966 Sep 9;153(3741):1269–1271. doi: 10.1126/science.153.3741.1269. [DOI] [PubMed] [Google Scholar]
  11. Whitfeld P. R., Spencer D. Buoyant density of tobacco and spinach chloroplast DNA. Biochim Biophys Acta. 1968 Apr 22;157(2):333–343. doi: 10.1016/0005-2787(68)90087-7. [DOI] [PubMed] [Google Scholar]
  12. Woodcock C. L., Fernández-Morán H. Electron microscopy of DNA conformations in spinach chloroplasts. J Mol Biol. 1968 Feb 14;31(3):627–631. doi: 10.1016/0022-2836(68)90435-x. [DOI] [PubMed] [Google Scholar]
  13. YOTSUYANAGI Y., GUERRIER C. MISE EN 'EVIDENCE PAR DES TECHNIQUES CYTOCHIMIQUES ET LA MICROSCOPIE 'ELECTRONIQUE D'ACIDE D'ESOXYRIBONUCL'EIQUE DANS LES MITOCHONDRIES ET LES PROPLASTES D'ALLIUM CEPA. C R Hebd Seances Acad Sci. 1965 Feb 22;260:2344–2347. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES