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. 1969 Mar;44(3):377–384. doi: 10.1104/pp.44.3.377

Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations 1

Stephen A Bard a, Milton P Gordon a
PMCID: PMC396095  PMID: 5775204

Abstract

A method for the large-scale preparation of spinach chloroplasts using the Spinco Model L-4 zonal ultracentrifuge and for the extraction of DNA from the chloroplasts is described. Thirty-five percent of the chloroplast DNA (ρ = 1.706 g/cc) differs from nuclear DNA (ρ = 1.695 g/cc) in buoyant density. Tm, base composition, and renaturation properties. Sixty-five percent of the chloroplast DNA (ρ = 1.696 g/cc) has the same buoyant density and Tm as nuclear DNA, but it differs in base composition and renaturation properties.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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