TABLE 1.

Effect of different stressors on survival (%) of exponentially growing S. cerevisiae YPH250 (wt) and YPH250 (Δyap 1) cells after pre-incubation with R. rosea aqueous extracts.

	Concentration of R. rosea aqueous extract, μl/ml medium
Stressor	0	1	5	20
YPH250
10 mM H2O2	61±7	81±7*	39±7*	39±6*
2 mM Cu2+	27±6	49±9*	28±7	22±6
2 mM Cu2+/4 mM H2O2	10±4	23±5*	17±2	16±3
20% C2H5OH	14±4	11±4	7±2	10±2
200 mM CH3COOH	65±7	80±10	78±12	91±5*
Heat shock (40°C)	54±5	70±7*	66±6	57±9
YPH250 (Δyap1)
10 mM H2O2	39±6	32±7	30±6	NS
Heat shock (40°C)	86±11	94±3	85±5	NS

Cells growing exponentially in YPD medium were pre-incubated with R. rosea aqueous extracts during 2 h, and then they were collected and resuspended in 50 mM K-phosphate buffer (pH 7.0) and exposed to different stressors for 1 h at 28°C, n=5–6.

^*Significantly different from respective control values with P<0.01 using Student’s t-test. Results are shown as means ± SEM (n = 4–6). NS – not studied.