TABLE 2.
Effect of hydrogen peroxide and heat shock on survival (%) of exponentially growing S. cerevisiae W303-1A (wt) and Wmsn2msn4 (Δmsn2Δ msn4) cells after pre-incubation with R. rosea aqueous extracts.
| Concentration of R. rosea aqueous extract, μl/ml medium
|
||||
|---|---|---|---|---|
| Stressor | Strain | 0 | 1 | 5 |
| 10 mM H2O2 | W303-1A (wt) | 83±1 | 95±5* | 49±10 |
| Wmsn2msn4 (Δmsn2Δ msn4) | 5±2 | 3±1 | 3±1 | |
| Heat shock (40°C) | W303-1A (wt) | 87±6 | 88±8 | 72±4 |
| Wmsn2msn4 (Δmsn2Δ msn4) | 57±12 | 85±8* | 76±12 | |
Cells growing exponentially in YPD medium were pre-incubated with R. rosea aqueous extracts during 2 h, and then they were collected by centrifugation and resuspended in 50 mM K-phosphate buffer (pH 7.0) and exposed to stresses for 1 h, n=5–6.
*
Significantly different from respective control values (without R. rosea extract) with P<0.01 using Student’s t-test. Results are shown as means ± SEM (n=5–6).