Fig. 3. An association between NEMO and IKKα is dispensable to regulate non-canonical NF-κB.

(A) WT and IKKα-deficient (IKKα^KO) MEFs retrovirally transduced with either MigR1 alone or MigR1 expressing IKKα^WT or IKKα^ΔNBD were incubated with LIGHT for the indicated times. Cell lysates were then analyzed by Western blotting with antibodies against the indicated proteins. (B) WT, IKKα^KO, and IKKα^KO MEFs transduced with the indicated constructs were incubated for 4 hours with LIGHT and then were treated either with cycloheximide (CHX) for the indicated times or with ethanol for 60 min as a vehicle control (V). Lysates were then analyzed by Western blotting with antibodies against the indicated proteins. Western blots are representative of four independent experiments.