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. Author manuscript; available in PMC: 2014 Mar 20.
Published in final edited form as: Oncogene. 2012 Jan 16;31(45):4798–4802. doi: 10.1038/onc.2011.635

Figure 3. NF-κB mediates the effects of FOXC1 on cell proliferation, migration, and invasion.

Figure 3

(A) FOXC1- or vector-overexpressing MDA-MB-231 cells were treated with the NF-κB inhibitor BMS-345541 (2 μM), followed by MTT assays (left), transwell migration assays (middle), and transwell invasion assays (right). Data represent mean ± SD (n = 3). (B) Wild-type (wt) and IKKα/IKKβ-null MEFs were transfected with FOXC1 or the vector, followed by cell proliferation MTT assays at the indicated time points (left). Deficiency of IKK expression in knockout MEFs is shown by immunoblotting (right). (C) Wild-type (wt) and IKKα/IKKβ-null MEFs were transfected with FOXC1 or the vector, followed by cell cycle analysis using flow cytometry (left). *, P < 0.05. CyclinD1 expression was assessed by immunoblotting (right).