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Histogram of a batch of normal β2AR cells (A) and β2AR cells requiring sorting (B). Notice the small proportion of the cells that appear to have no surface expression of FAP-β2AR cells in R2 of Figure 4A. This phenomenon is commonly observed in all FAP-GPCR cells we have worked with and will not affect the cell function/experimental result. However, the cells will require sorting if a significant proportion of the cells appear to lose surface expression as indicated in Figure 4B.
