Figure 8.

Flow cytometric analysis of apoptotic RPMI-8226 cells for 24 hours. RPMI-8226 cells treated for indicated time periods with 0 mg/L, GO 50 mg/L, DOX 2 mg/L, and GO/DOX (GO 50 mg/L + DOX 2 mg/L). Cells were harvested, promptly stained with annexin V-FITC, and analyzed on a flow cytometer (Cytomic™ FC500; Beckman Coulter, Miami, FL, USA). Scattergrams from a representative experiment are shown.

Abbreviations: DOX, doxorubicin; FITC, fluorescein isothiocyanate; GO, graphene oxide; GO/DOX, GO loaded with DOX.