Figure 4.

A, current traces recorded after incubation of cells with 10 mm βCD (90 min). B, current traces obtained after co-incubation of cells with 10 mm βCD and 50 μg ml⁻¹ LPS (90 min). C, mean V_0.5 recorded under control conditions (open bar, n = 15), in the presence of 50 μg ml⁻¹ LPS (black bar, n = 8), after incubation of cells with 10 mm βCD (60–120 min, light grey bar, n = 10) and after co-incubation of cells with 10 mm βCD and 50 μg ml⁻¹ LPS (60–120 min, dark grey bar, n = 13). βCD shifts V_0.5 of I_hHCN2 to less negative potentials. However, in the presence of βCD, LPS does not modulate I_hHCN2. *P < 0.05 versus control.