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Representative Western blots of whole muscle lysates (Homog.) and isolated mitochondria (Mito.) showing the presence of known mitochondrial proteins and the absence of plasma membrane (caveolin-1) and sarcoplasmic reticulum (SERCA) proteins. Mitochondrial proteins included the E1α subunit pyruvate dehydrogenase (PDHE1α) and subunits of complex 3 and 4 of the electron transport chain. These were loaded as 30 μg of whole muscle and 5 μg of mitochondria.
