Fish oil supplementation results in changes in omega-3 (A) and omega-6 (B) PUFA content in isolated mitochondrial membranes of human skeletal muscle. The content of α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), linoleic acid (LA) and arachidonic acid (AA) are reported in various phospholipid species, including: phosphatidylcholine (PC), phosphatidylethanolamine (PE), cardiolipin (CL), sphingomielin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS). Values represent means ± SEM, and are expressed as a percentage of pre-supplementation. n = 9. *Significantly (P < 0.05) different from pre-supplementation. ND; Not detected.