Omega-3 incorporation into mitochondrial membranes does not alter maximal mitochondrial respiration (A), the expression of oxidative phosphorylation (OXPHOS) proteins (B), mitochondrial pyruvate kinetics, (C) or the apparent Km for pyruvate (D). Values were compared before (Pre) and after (Post) 12 weeks of omega-3 supplementation. Non-ADP-stimulated (pyruvate + malate; PM), ADP-stimulated (PMD), maximal complex I supported respiration (PMDG) and maximal coupled respiration (PMDGS) were not different after supplementation. Values represent means ± SEM. n = 18.