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. 2014 Feb 13;592(Pt 6):1341–1352. doi: 10.1113/jphysiol.2013.267336

Figure 4.

Figure 4

Omega-3 incorporation into mitochondrial membranes altered ADP respiratory kinetics in the presence (A, B) and absence (C, D) of creatine. Michaelis–Menten kinetic curves were generated in the presence (A) and absence (C) of creatine. The ADP concentration required to elicit half-maximal respiration (apparent Km) was determined in the presence (B) and absence (D) of creatine. Values were compared before (Pre) and after (Post) 12 weeks of omega-3 supplementation. Fibre bundles were recovered from the respiration chamber and used to determine the expression of oxidative phosphorylation (OXPHOS) proteins (E). The protein content of adenine nucleotide translocase (ANT1, ANT2) was determined by Western blotting (F). Values represent means ± SEM. n = 9–18. *Significantly (P < 0.05) different from Pre.