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. 2014 Mar 4;2:e280. doi: 10.7717/peerj.280

Table 1. Primers developed and used in this study.

Gene Gen bank/Ensembl sequences Primers (5′–3′) (q = qPCR primers based on koala sequence) TAnnealing (°C) Amplicon size (bp) Proportion of full length coding region amplified (%)
CD4 Saha XM_003771252.1 CD4 F: GTGTTCAAGGTGACAGCCA 56–50 525 40.3
Maeu EF490599.1 CD4 R:AAGACTCCTGAGCCAGCA
Modo NM_001099290.1 qCD4 F: GCCAACCCAAGTGACTCTGT 60 104
qCD4 R: TCTCCTGGACCACTCCATTC
CD8β Saha XM_003758647.1 CD8 F: ACCCACCAAAAAGACCAC 65–50 359 36.2
Maeu EU152105.1 CD8 R: ACCCCAGTGGCTTAGGAAAA
Modo NM_001146331.1 qCD8 F: ATGTAGCTCGGCACCACTTT 60 159
qCD8 R: TTGCTGTTCTCATGCCTCAG
IL-4 Saha XP_003756539.1 IL4 F: GTCTCACATTCCAACTG 56–43 417 89.2
Maeu ADG01643.1 IL4 R: TGCTCAGGAAATCTTGC
Modo XP_003339570.1 qIL4 F: GTTTCCCTGCTTTGAGATGG 55 78
qIL4 R: TCAGGAAACAGCTTCGGAGT
IL-6 Saha ENSSHAT00000013262 IL6 intron F: GCAAAGGGAAACTCACCA 65–50 426 22.0
Modo ENSMODT00000001552 IL6 intron R: AACACCTGTTTGGCTTTTAG
qIL6 F: TGGATGAGCTGAACTGTACCC 60 118
qIL6 R: GCTTGCCAAGGATTGTGAGT
IL-10 Trvu AF026277.1 IL10 F: ACATGCTCCGAGAWCTTCG 56–43 351 65.2
Saha XM_003767646.1 IL10 R: TGTAGACTCCTTGTTCC
Modo XM_003340167.1 qIL10 F: TTTAGGCGAGAAGCTGAAGG 55 69
qIL10 R: TCTTCACAGGGCAGGAATCT
IFNγ Saha ENSSHAT00000017850 IFNγ F: CAAGCTNCNTCTTAGCATCC 65–50 466 84.3
Maeu ENSMEUT00000007026 IFNγ R: TGGCTTNTGTTCTGTCTTC
Modo AC190119.3 qIFNγ F: TGAACATGATGGATCGTTGG 60 186
qIFNγ R: CATTCACTTTGCTGGCAGTG
GAPDH Saha XM_003771214.1 GAPDH F: AGTCCACTGGCGTGTTTACC 55 549
Modo EF599650.1 GAPDH R: GGTCCTCTGTGTAGCCCAAG
qGAPDH F: AACTTTGGCATTGTGGAAGGA 55 130
qGAPDH R: AACATCATCCCTGCTTCCAC