Figure 6. dPdxk¹ mutations cause nucleotide unbalance but are only weakly sensitive to hydroxyurea (HU).

(A) dPdxk¹ mutant larvae have a very low dTTP content and accumulate uracil; they also exhibit a reduced PLP level. Values are the means of three independent experiments (±SD). * and **, indicate significant differences from wild type controls with p<0.05 and p<0.01, respectively. (B) dPdxk¹ mutants are weakly sensitive to HU. In HU-treated (2 mM) dPdxk¹ mutant brains, 1.6 % of metaphases exhibited shattered chromosomes; these metaphase were rarely observed (0.3%) in control and dPdxk¹ untreated mutant brains. Each bar in the graph represents the mean frequency of CABs (±SE) obtained by examining at least 600 metaphases form at least 6 brains.