Figure 2. dATP8B is required for responses of Or but not IR or Gr- expressing sensory neurons.

(A–C) Mutations in dATP8B reduce the sensitivity of Or-expressing neurons to their major ligands. Dose-response curves for neurons located in three different morphological types of sensilla are shown; (A) ab3B neurons in basiconic sensilla (Or85b) to 2-heptanone, (B) at1A neurons in trichoid sensilla (Or67d) to cis-vaccenyl acetate, (C) ac3B neurons in coeloconic sensilla (Or35a) to Z3-hexenol. In all cases sensitivity is significantly lowered for all doses in homozygous dATP8B^f05203 flies (red) compared to controls (black, t-test, Bonferroni, n = 6–9, recorded from 4–8 flies). (D–E) Mutations in dATP8B differentially alter responses of ab3A neurons (Or22a) to a major ligand ethyl hexanoate (D) and a minor ligand ethyl butanoate (E). (n = 7 sensilla, recorded from 5–6 flies). (F–I) Neurons expressing IR or Gr receptors are not affected by dATP8B mutations. Four different types of neurons are shown; (F) responses of ac2A neurons in coeloconic sensilla (IR41a and IR76b) to 1,4-diaminobutane, (G) responses of ac3A neurons in coeloconic sensilla (IR75a,b and c) to propionic acid, (H) responses of ab1C neurons in basiconic sensilla (Grs21a and 63a) to CO₂, (I) responses from single neurons in labellar taste sensilla expressing Gr genes to 100 mM sucrose (Suc) or 10 mM caffeine (Caff) (mean ± SEM). In all cases control and mutant responses are not significantly different (n = 6–10 sensilla from 3–5 flies, t-tests, Bonferroni). Controls are either wild type or heterozygous dATP8B^f05203 mutants.