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. 2014 Mar 20;10(3):e1004218. doi: 10.1371/journal.pgen.1004218

Figure 5. UVR8 mutations affect UV-B light perception, UVR8 monomerization and UVR8-COP1 association.

Figure 5

(A) Absorbance at 310 nm of plant total proteins extracted from 4-day-old seedlings grown under −UV-B and +UV-B. Data are shown as mean ± SD; n = 3. *, p<0.05. **, p<0.01. Student's t test. (B) Conformational status of wild-type and mutated UVR8 proteins in 4-day-old seedlings grown under −UV-B and +UV-B. Total plant proteins without heat denaturation were assayed in SDS-PAGE and immunoblot analysis by anti-GFP antibody. Anti-RPN6 was used as a loading control. The asterisks indicate unspecific degradation products. (C) In vivo co-immunoprecipitation (co-IP) assays using 4-day-old seedlings grown under −UV-B and +UV-B by anti-GFP antibody. Immunoblot analysis was performed by anti-GFP and anti-COP1 antibodies. Anti-RPN6 was used as a un-immunoprecipitated and loading control.