Figure 4. Reduced expression of heterochromatic genes in Lhr and Hmr mutants.

Loss of Lhr (A) and Hmr (B) leads to a statistically significant reduction in the expression of heterochromatic genes. Significance of difference was calculated using the Wilcoxon rank sum test with continuity correction (for (A) p = 3.549e-05, for (B) p = 1.461e-09). Box plots show log₂ fold change of 7838 euchromatic and 370 heterochromatic genes for (A) and 7451 euchromatic and 344 heterochromatic genes for (B). The definition of the euchromatin-heterochromatin boundary for all chromosomes comes from experiments done in S2 tissue culture cells, except for 3R, which comes from the cytogenomic border [100].