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. 2014 Mar 20;10(3):e1004240. doi: 10.1371/journal.pgen.1004240

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© 2014 Satyaki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) sim-Hmr-HA colocalizes with HP1a (red) in nuclear cycle 14 D. melanogaster Hmr³; sim-Hmr-HA embryos. The sim-Hmr-HA transgene was transformed into D. melanogaster at the identical attP2 site used for mel-Hmr-HA above (Figure 1). DAPI is shown in blue. (B) mel-Hmr-FLAG does not fully complement TE derepression in Hmr⁻. 9 TE families are 2–9× more highly expressed in Hmr⁻; ø{mel-Hmr-FLAG}/+ compared to Hmr^+/−. (C) Comparison of TE expression in Hmr⁻; ø{mel-Hmr-FLAG}/+ and Hmr⁻; ø{sim-Hmr-FLAG}/+. For B and C, reads were mapped to the individual-insertion database. TEs are considered differentially expressed in the pairwise comparisons if there was at least a 2× fold change and p<0.001.