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. 2014 Mar 20;8(3):e2746. doi: 10.1371/journal.pntd.0002746

Figure 3. Expression of Gn/Gc in Vero-G cells.

Figure 3

(A) Vero E6 cells were infected with MP-12 at an MOI of 1 and cell extracts were prepared at 12 h p.i. (MP-12). The same amounts of cell extracts were prepared from Vero-G cells (Vero-G). Both extracts were subjected to Western blot analysis by using anti-MP-12 antibody. (B) Subcellular localization of Gn/Gc in Vero-G cells. Mock-infected Vero E6 cells (Vero E6 cells), MP-12-infected Vero E6 cells and Vero-G cells were fixed, treated with Triton X-100 (permeabilized) or left without treatment (non-permeabilized) and stained with anti-Gn (Gn) or anti-Gc (Gc) monoclonal antibodies.