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. 2014 Mar 20;9(3):e89792. doi: 10.1371/journal.pone.0089792

Figure 6. Cbl-b is required for the endocytic transit of TLR9.

Figure 6

(A) Representative confocal microscopic images of splenocytes from mice with indicated genotypes. For experiments, cells were stimulated through the BCR (green) for 30 minutes then fixed and stained for TLR9 (blue) and Lamp-1 (red)(n = 3). (B) Quantitation of experiments shown in (A) for fraction of cells demonstrating significant co-localization of TLR9 with Lamp-1 (n = 3, 30 cells/exp) (Cbl-b−/− vs. CblbC373A or WT, p<0.001). (C) Quantitation of fractions of cells in (A) demonstrating significant co-localization between BCR and TLR9 (n = 3, 30 cells/exp). (D) In vitro assay of T-bet induction in response to ODN 1826 or control ODN targeted through the BCR (n = 3, p<0.01).